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Enhong Ouyang

Determination of 5-hydroxymethyl-2-furaldehyde of crude and processed fructus corni in freely moving rats using in vivo microdialysis sampling and liquid chromatography

Enhong Ouyang, Chengrong Zhang, Xiaomeng Li

Pharmacognosy Magazine 2011 7(28):271-276

Background: Fructus Corni is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc. 5-hydroxymethyl-2-furaldehyde (5-HMF) is an important active composition of the Fructus Corni. However, there have been no reports on the concentration of 5-HMF in freely moving rats using microdialysis coupled with HPLC. Materials and Methods: The concentration of 5-HMF in free-moving rats after intra-gastric (i.g.) administration of the water extract of Fructus Corni and JZP was analyzed by microdialysis coupled with high-performance liquid chromatographic (HPLC). Results: Results demonstrated that the concentration of 5-HMF in microdialysate was 1.4951 μg/l, but higher in rat microdialysate after i.g. administration of the aqueous extract of JZP (5.2662 μg/l). Conclusion: This method is proved to be rapid, accurate and simple. Real-time in vivo monitoring the concentration of 5-HMF provides the theoretical basis for further explaining the processing mechanism of Fructus Corni.

Simultaneous determination of geniposide, chlorogenic acid, crocin1, and rutin in crude and processed fructus gardeniae extracts by high performance liquid chromatography

Enhong Ouyang, Chengrong Zhang, Xiaomeng Li

Pharmacognosy Magazine 2011 7(28):267-270

Background: Fructus Gardeniae, commonly used traditional Chinese medicine (TCM) called Zhizi in chinese, is derived from the dried fruit of Gardenia jasminoides Ellis of the Madder Family. To our knowledge, previously reported analytical methods were not developing for simultaneous determination of geniposide, chlorogenic acid, crocin1, and rutin in Fructus Gardeniae and its processed products of chaozheng pin (CZP) extracts. Materials and Methods: In this study, a HPLC method was developed for simultaneous determination four major active components in Fructus Gardeniae and its processed products. Results: The contents of geniposide, chlorogenic acid, crocin1, and rutin in Fructus Gardeniae samples analyzed were 2.492 – 4.242%, 0.162 – 0.407%, 0.417 -0.837%, and 0.116 – 0.251%, respectively. Conclusion: The developed method can be applied to the intrinsic quality control of Fructus Gardeniae.

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