Miyoung Kim, Hanna Kim, Jeonghyun Ryu, Suzy Jo, Guemsan Lee, Mi Heon Ryu, Hyungwoo Kim, Su In Cho

Pharmacognosy Magazine 2014 10(38):377-382

Background: The slough shed of Cryptotympana atrata Fabricius is widely used to treat skin diseases in China, Japan, and Korea. Objective: To investigate the anti-inflammatory effects of C. atrata on contact dermatitis. Materials and Methods: We investigated the effects of C. atrata methanol extract (MECA) on ear swelling, histophathological changes and cytokine production in 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) mice. Results: Topical application of MECA effectively inhibited enlargement of ear swelling (30 and 100 &#956;/ear, P < 0.05; 300 &#956;g/ear, P < 0.01). MECA treatment also inhibited hyperplasia, spongiosis (100 and 300 &#956;g/ear, P < 0.001), and immune cell infiltration (30 &#956;g/ear, P < 0.05; 100 and 300 &#956;g/ear, P < 0.001) induced by DNFB. In addition, treatment with MECA suppressed the increase in the levels of TNF-&#945; (P < 0.05), IFN-g (3, 100 &#956;g/ear, P < 0.05; 300 &#956;g/ear, P < 0.01), and IL-6 (100 &#956;g/ear, P < 0.05; 300 &#956;g/ear, P < 0.01) production. Conclusion: These data suggest that MECA has the potential for use in the treatment of inflammatory skin diseases, including CD. Moreover, the results presented herein indicate that anti-inflammatory actions of MECA are mediated by decreasing production of TNF-&#945;, IFN-&#947;, and IL-6 in inflamed tissues.